Catalog Number - 68508-10P-2

Cogent RP C8™ HPLC Columns are designed for reliable reversed-phase chromatography using a traditional octyl (C8) bonded stationary phase. Manufactured with ultra-high purity silica and fully double end capped bonding technology, these columns provide reproducible retention, excellent peak shape, and long column life. The RP C8™ phase is a USP L7 equivalent chemistry and is commonly used as an alternative for methods developed on similar C8 phases. Compared to C18 columns, RP C8™ generally provides lower hydrophobic retention while maintaining excellent selectivity for many neutral and non-polar compounds.

68508-10P-2 is packed with 5 µm RP C8™ particles featuring a 100 Å pore size in a 2.1 mm ID x 100 mm length format. The 100 mm column length provides increased resolving power compared to shorter 50 mm columns while maintaining reasonable analysis times. The RP C8™ stationary phase is well suited for hydrophobic compounds, neutral analytes, and long-chain fatty acids where somewhat lower retention than a comparable C18 column is desired. The 5 µm particle size provides low backpressure and broad compatibility with conventional HPLC and LC-MS systems.

This column is frequently selected for LC-MS applications when greater chromatographic resolution is required without moving to longer analytical columns. The 2.1 mm ID format minimizes solvent consumption and is particularly suited for mass spectrometry workflows. Optimum performance is typically achieved at approximately 0.2-0.3 mL/min. Standard HPLC systems used with this column must be capable of delivering accurate and reproducible low flow rates, and low-volume detector flow cells are recommended to minimize extra-column band broadening.

Key Benefits

• RP C8™ stationary phase

• USP L7 equivalent chemistry

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 100 Å pore size

• Increased resolution compared to 50 mm columns

• Lower retention than C18 phases

• Reduced solvent consumption

• Excellent for LC-MS applications

Typical Applications

• Reversed-phase HPLC

• LC-MS analysis

• Method development

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Hydrophobic compound separations

• Neutral compound analysis

• Environmental testing

• Bioanalytical methods

• Routine quality control

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™

• USP Classification: L7

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Recommended Flow Rate: 0.2-0.3 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for reversed-phase HPLC and LC-MS applications requiring increased resolution

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for long-chain fatty acid separations

• 100 mm length provides greater resolving power than shorter analytical columns

• 5 µm particles provide low backpressure and broad instrument compatibility

• Requires an HPLC system capable of precise and reliable low flow rate delivery

• Low-volume detector flow cells are recommended to maximize efficiency

• Reduces solvent consumption compared to standard 4.6 mm ID columns

• Manufactured using ultra-high purity silica and fully double end capped bonding technology